Effect of Soybean Trypsin Inhibitor on Digestive Proteases and Growth of Larval Spodoptera Littoralis (boisd.)
نویسنده
چکیده
Two serine proteases were identified in midgut of 4 instar of Spodoptera littoralis. A trypsin-like one (BApNAhydrolyzing) (TRYP) with a pH optimum at 11.0, and a chymotrypsin esterase-like enzyme (BTEE-hydrolyzing) (CHYMO) with optimum activity at pH 10.0. Serine protease activities were significantly (p < 0.001) inhibited in vitro by soybean trypsin inhibitor (SBI). The incorporation of different concentrations of SBI into an artificial diet, consumed by the larvae, causes variable in vivo inhibitory effects. These effects were more prominent in the fourth instar; however, in the fifth instar the proteases activity recoverd again. The ingestion of dietary SBI also reduces the weight gain and survival of the larvae.
منابع مشابه
Protease purification and characterization of a serine protease inhibitor from Egyptian varieties of soybean seeds and its efficacy against Spodoptera littoralis
Serine inhibitors have been described in many plant species and are universal throughout the plant kingdom. Trypsin inhibitors are the most common type. In the present study, trypsin and chymotrypsin inhibitory activity was detected in the seed flour extracts of four Egyptian varieties of soybean (Glycine max). The soybean variety, Giza 22, was found to have higher trypsin and chymotrypsin inhi...
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